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Toxicol. Sci. 85, 727-734. Inhibitory effects of vitamin A on TCDD-induced cytochrome P-450 1A1 enzyme activity and expression. 2005

Yang, Y.M., Huang, D.Y., Liu, G.F., Zhong, J.C., Du, K., Li, Y.F. and Song. X.H.

Notes: The ability of the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to upregulate cytochrome P450 CYP1A1 levels in mouse liver was examined. Total mouse liver RNA was reverse transcribed using the Reverse Transcription System, and the resulting CYP1A1 cDNA was quantitated using real-time PCR. CYP1A1 protein levels were quantitated by Western blot using an anti-CYP1A1 antibody, the Anti-Rabbit IgG (Fc), Alkaline Phosphatase Conjugate secondary antibody and the Western Blue® Stabilized Substrate for Alkaline Phosphatase.

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J. Biol. Chem. 274, 30919-30926. Interleukin-6 increases rat metalloproteinase-13 gene expression through stimulation of activator protein 1 transcription factor in cultured fibroblasts. 1999

Solis-Herruzo, J.A., Rippe, R.A., Schrum, L.W., de la Torre, P., Garcia, I., Jeffrey, J.J., Munoz-Yague, T., Brenner, D.A.

Notes: Extracts were made from Rat-1 fibroblasts with or without IL-6 treatment. Lysates were prepared with Dignam C buffer minus the phosphatase inhibitors. The extracts were run over a G-25 spin column to remove free phosphate and were then assayed using the Serine/Threonine Phosphatase Assay System. Western Blue® Stabilized Substrate for Alkaline Phosphatase was used for developing Western blots. (0350)

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Proc. Natl. Acad. Sci. USA 96, 2970-2975. Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene. 1999

Drotschmann, K., Clark, A.B., Tran, H.T., Resnick, M.A., Gordenin, D.A., Kunkel, T.A.

Notes: Immunoblots, on PVDF, were developed with the Anti-Rabbit IgG Alkaline Phosphatase Conjugate and the Western Blue® Stabilized Substrate for Alkaline Phosphatase. (1200)

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J. Biol. Chem. 274, 12650-12655. Vesicular ATPase-overexpressing cells determine the distribution of malaria parasite oocysts on the midguts of mosquitoes. 1999

Cociancich, S.O., Park, S.S., Fidock, D.A., Shahabuddin, M.

Notes: The Western Blue® Stabilized Substrate for Alkaline Phosphatase was used to develop immunoblots on nitrocellulose membranes. (1312)

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Biotechnol. Appl. Biochem. 28, 125-131. Recombinant human mast cell tryptase beta: stable expression in Pichia pastoris and purification of fully active enzyme. 1998

Niles, A.L., Maffitt, M., Haak-Frendscho, M., Wheeless, C.J., and Johnson, D.A.

Notes: Human mast cell tryptase was expressed and purified from Pichia pastoris by hydrophobic interaction chromatography followed by affinity chromatography on immobilized heparin. A faint band at 33Da and diffuse bands at 34.2, 35.9 and 50 kDa were observed on SDS/PAGE due to differences in the post-translational glycosylation of asparagine residues. Expression patterns of human tryptase were analysed by separating proteins by 10% SDS/PAGE , transferring to nitrocellulose membrane, and immunoblotting with the Anti-Human Tryptase mAb Biotin at 100 ng/ml in TBST. The blots were incubated with a 1:5000 dilution of Promega's Anti-Mouse IgG (H+L), AP Conjugate and developed with the Western Blue® Stabilized Substrate for Alkaline Phosphatase. (2451)

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