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Promega Corporation

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Proc. Natl. Acad. Sci. USA 101(7), 2112-2116. DNA-dependent PK inhibits adeno-associated virus DNA integration. 2004

Song S., Lu Y., Choi Y.K., Han Y., Tang Q., Zhao G., Berns K.I. and Flotte T.R.

Notes: Using an in vitro model of recombinant adeno-associated virus (rAAV) integration, the authors examined if the presence or absence of DNA-Dependent Protein Kinase affected AAV integration. (3081)

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J. Biol. Chem. 274, 478-485. Nuclear extracts lacking DNA-dependent protein kinase are deficient in multiple round transcription. 1999

Woodard, R. L. , Anderson, M. G. , Dynan, W. S.

Notes: The SignaTECT® DNA-Dependent Protein Kinase Assay System was used to confirm the inhibitory effect of Wortmannin on the kinase activity of DNA-Dependent Protein Kinase (DNA-PK). (0152)

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Nature 394, 700-704. DNA-dependent protein kinase acts upstream of p53 in response to DNA damage. 1998

Woo, R.A., McLure, K.G., Lees-Miller, S.P., Rancourt, D.E. and Lee, P.W.

Notes: Promega's SignaTECT® DNA-Dependent Protein Kinase Assay System was used in this study. (1962)

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J. Biol. Chem. 273, 1568-1573. Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells. 1998

Bandyopadhyay, D., Mandal, M., Adam, L., Mendelsohn, J. and Kumar, R.

Notes: The authors used DNA-dependent Protein Kinase (DNA-PK) and substrate with several human carcinoma cell lines (1459)

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J. Biol. Chem. 273, 25654-25658. Regulation of DNA-dependent protein kinase by the Lyn tyrosine kinase. 1998

Kumar, S., Pandey, P., Bharti, A., Jin, S., Weichselbaum, R., Weaver, D., Kufe, D., Kharbanda, S.

Notes: The authors used Promega's purified DNA-Dependent Protein Kinase as a substrate for in vitro phosphorylation by the protein tyrosine kinase Lyn. They also used the TNT® Coupled Reticulocyte Lysate System to synthesize radiolabeled DNA-dependent protein kinase (DNA-PK) catalytic subunit polypeptides for protein:protein interaction studies with GST-tagged Lyn. (0863)

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J. Biol. Chem. 273, 1794-1801. The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase. 1998

Leber, R. , Wise, T. W. , Mizuta, R. , Meek, K.

Notes: The authors used Promega's DNA-dependent Protein Kinase (DNA-PK) and Recombinant Human AP1 for in vitro c-Jun kinase assays (0805)

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J. Biol. Chem. 272, 5647-5658. Sequence-specific DNA binding and transcription factor phosphorylation by Ku Autoantigen/DNA-dependent protein kinase. Phosphorylation of Ser- 527 of the rat glucocorticoid receptor. 1997

Giffin, W., Kwast-Welfeld, J., Rodda, D.J., Prefontaine, G.G., Traykova-Andonova, M., Zhang, Y., Weigel, N.L., Lefebvre, Y.A., Hache, R.J.

Notes: The DNA-Dependent Protein Kinase (DNA-PK) was used to phosphorylate a GST-glucocorticoid receptor fusion protein. (1104)

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J. Biol. Chem. 272, 23896-23904. Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells 1997

Zernik-Kobak, M. , Vasunia, K. , Connelly, M. , Anderson, C. W. , Dixon, K.

Notes: DNA-Dependent Protein Kinase (DNA-PK) was used to in vitro phosphorylate replication protein A. The phosphorylation was compared to replication protein A isolated directly from HeLa cells. Many details of the reaction are provided. (0086)

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J. Biol. Chem. 272, 22191-22198. SV40 large tumor antigen nuclear import is regulated by the double- stranded DNA-dependent protein kinase site (Serine 120) flanking the nuclear localization sequence 1997

Xiao, C. Y. , Hubner, S. , Jans, D. A.

Notes: Promega's Casein Kinase II and DNA-Dependent Protein Kinase were used for in vitro phosphorylation of the SV40 large T antigen expressed either from Rabbit Reticulocyte Lysate or a large Tantigen-β-Gal fusion protein expressed in a rat hepatoma cell line. Lysates and cell extracts were assayed for DNA-dependent protein kinase activity with a peptide substrate. The ability of β-Gal-T antigen fusions (± phosphorylation) to bind to murine importin subunits was assessed by an ELISA system using the Anti-β-Galactosidase mAb. (0120)

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