Sawamoto, K., Winge, P., Koyama, S., Hirota, Y., Yamada, C., Miyao, S., Yoshikawa, S., Jin, M.H., Kikuchi, A., and Okano, H.
Notes: A Ral GTPase, which regulates developmental changes through inhibition of the JNK pathway, is identified in Drosophila. Inhibition of the JNK pathway in the presence of a constitutively active Ral protein was examined by Western blot using Promega's Anti-ACTIVE® JNK pAb. Drosophila S2 cells were treated with 500 mM sorbitol for 5 min, lysed, subjected to SDS-PAGE, and transferred to nylon membranes. The membranes were incubated with Anti-ACTIVE® JNK to detect the dually phosphorlyated (active) form of JNK. (2385)