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J. Biol. Chem. 274, 19699-19706. Identification and characterization of a ran gene promoter in the protozoan pathogen Giardia lamblia 1999

Sun, C.-H., Tai, J.-H.

Notes: The pSP-luc+ Vector was used as a source of luciferase cDNA for construction of a expression vector in G. lamblia. The promoter of interest was combined with luc+ in a standard cloning vector for study. Deletion mutants of the promoter were constructed with the Erase-a-Base® System. The authors note that the pGL3 Control Vector produced some luciferase activity in the G. lamblia but only 0.5% of the RAN promoter-luc+ construct. (0317)

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