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Blood 90, 1751-1767. Development of a candidate HLA A*0201 restricted peptide-based vaccine against human cytomegalovirus infection. 1997

Diamond, D.J. , York, J. , Sun, J.Y. , Wright, C.L. , Forman, S. J.

Notes: Fragments of the pp65 protein cDNA were subcloned into a modified pCI-neo Mammalian Expression Vector containing an enterokinase cleavage site and 6XHis tag. These fusion vectors were co-transfected into COS cells with an HLA A*0201 gene-expressing pCI-Neo Vector construct. The transfected cells were assayed for TNFα production. TNFalpha was quantified by its cytotoxicity to WEHI-164 cells using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (1231)

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J. Biol. Chem. 272, 14227-14235. Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure. 1997

Smith, C. L., Htun, H., Wolford, R. G , Hager, G. L.

Notes: Several proteins were transiently expressed in the 1471.1 cell line using the pCI Mammalian Expression Vector. (0379)

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J. Biol. Chem. 272, 30866-30872. Dimerization Properties of Human BAD. Identification of a bh-3 domain and analysis of its binding to mutant bcl-2 and bcl-xl proteins 1997

Ottilie, S., Diaz, J.L., Horne, W., Chang, J., Wang, Y., Wilson, G., Chang, S., Weeks, S., Fritz, L.C., Oltersdorf, T.

Notes: The pCI-neo Mammalian Expression Vector was used to express the 168 amino acid BAD protein in 293 cells. Cells expressing the BAD protein were used for apoptosis studies. (0573)

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J. Biol. Chem. 272, 22776-22780. Glucose Catabolism in Cancer Cells. The type ii hexokinase promoter contains functionally active response elements for the tumor suppressor p53. 1997

Mathupala, S.P., Heese, C., Pedersen, P.L.

Notes: Luciferase studies were performed in the AS-30D hepatoma cell line using constructs made in the pGL2 Basic Vector. The effect of p53 on the hexokinase promoter was assayed in vivo by expression of the p53 from the pCI-neo Mammalian Expression Vector and promoter activity was reported with luciferase. All transfections were normalized to β-Galactosidase activity supplied by the pSV-β-Galactosidase Control Vector. (0700)

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Proc. Natl. Acad. Sci. USA 94, 8208-8213. Interaction between amyloid precursor protein and presenilins in mammalian cells: Implications for the pathogenesis of Alzheimer disease 1997

Xia, W. , Zhang, J. , Perez, R. , Koo, E. H. , Selkoe, D. J.

Notes: The pCI-neo Mammalian Expression Vector was used in a rather unique way. The neo gene was replaced with a transferrin receptor cDNA and another protein was subcloned downstream of the CMV promoter. Both proteins were expressed in CHO cells. Stable transfectants were obtained by cotransfection with the vector pZeo (Invitrogen) expressing yet a third protein. (0164)

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Oncogene 15, 2277-2287. JNK1, JNK2 and JNK3 are p53 N-terminal serine 34 kinases. 1997

Hu, M.C., Qiu, W.R., Wang, Y.P.

Notes: All three isoforms of JNK were subcloned into the pCI-neo Mammalian Expression Vector and a hemagglutinin tag. Expression vectors were also constructed with HPK1 and MEKK1 using the pCI-neo Mammalian Expression Vector. Constructs were expressed in 293T cells. Extracts of cells expressing either of the constructs were assayed for the ability to phosphorylate a p53-GST fusion protein. Expressed proteins were assayed for p53 interactions by co-expression followed by immunoprecipitation (1020)

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J. Biol. Chem. 272, 14459-14464. K- and N-Ras are geranylgeranylated in cells treated with farnesyl protein transferase inhibitors. 1997

Whyte, D. B. , Kirschmeier, P. , Hockenberry, T. N. , Nunez-Oliva, I. , James, L. , Catino, J. J. , Bishop, W. R. , Pai, J. K.

Notes: The mevalonate transporter protein gene was cloned into the  pCI-neo Mammalian Expression Vector and stably transfected into the DLD-1 cell line. Two mutant K-Ras protein genes and one mutant N-ras protein gene were cloned into the  pCI-neo Mammalian Expression Vector and transiently expressed in NIH3T3 cells and COS-7 cells. (0183)

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J. Biol. Chem. 272, 19777-19784.. Localization of the major NFκB-activating site and the sole TRAF3 binding site of LMP-1 defines two distinct signaling motifs. 1997

Brodeur, S.R., Cheng, G., Baltimore, D., Thorley-Lawson, D.A.

Notes: The pCI-Neo Mammalian Expression Vector was used in conjunction with a luciferase reporter vector to determine the important regions on the carboxy-terminal portion of the 386-amino acid LMP-1 protein. Both full-length and truncation mutants were expressed in the BJAB human EBV-negative lymphoblastoid B cell line. Luciferase activity was measured using the Luciferase Assay System. (1409)

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J. Biol. Chem. 272, 8957-8961. Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase. 1997

Spicer, A. P., Olson, J. S., McDonald, J. A.

Notes: The pCI-neo Mammalian Expression Vector was used to express a 554-amino acid hyaluronan synthase in COS-1 cells. (0363)

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J. Neurosci. 17, 6534-44. Neural agrin induces ectopic postsynaptic specializations in innervated muscle fibers. 1997

Meier, T., Hauser, D.M., Chiquet, M., Landmann, L., Ruegg, M.A., Brenner, H.R.

Notes: A fusion protein of the extracellular domain of the tyrosine kinase receptor Nsk2 was fused to the Fc portion of mouse immunoglobulin and subcloned into the pCI-neo Mammalian Expression Vector. The construct was stably expressed from 293 cells. The protein was recovered from the conditioned media by Protein A affinity chromatography. The protein was used to create an anti-Nsk2 antisera. (0676)

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J. Biol. Chem. 272, 24371-24379. New Modulatory alpha Subunits for Mammalian Shab K+ Channels 1997

Salinas, M., Duprat, F., Heurteaux, C., Hugnot, J.P., Lazdunski, M.

Notes: The pCI Mammalian Expression Vector was used to express a flag-tagged 497 amino acid potassium channel in COS cells. (0433)

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J. Biol. Chem. 272, 24363-24370. p32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation. 1997

Muta, T., Kang, D., Kitajima, S., Fujiwara, T. and Hamasaki, N.

Notes: The pCI-neo Mammalian Expression Vector was used to transiently express the 282 amino acid p32 protein and a 73 N-terminal amino acid truncation in PCL cells. Cells were assayed by Western blotting and immunocytochemistry. Transfections were performed with the Transfectam® Reagent. (0636)

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EMBO J. 16, 5472-5479.. Properties of KvLQT1 K+ channel mutations in Romano-Ward and Jervell and Lange-Nielsen inherited cardiac arrhythmias 1997

Chouabe, C. , Neyroud, N. , Guicheney, P. , Lazdunski, M. , Romey, G. , Barhanin, J.

Notes: The 676 amino acid KvLQT1 protein and site-directed mutants were cloned into the pCI Mammalian Expression Vector, expressed in COS cells and assayed for activity. (1297)

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J. Biol. Chem. 272, 16955-16961. Structural and functional complementation of an inactive bcl-2 mutant by bax truncation 1997

Ottilie, S., Diaz, J.L., Chang, J., Wilson, G., Tuffo, K.M., Weeks, S., McConnell, M., Wang, Y., Oltersdorf, T., Fritz, L.C.

Notes: The pCI-neo Mammalian Expression Vector was used to express three proteins, bcl-2, bcl-2(G145A), and Bax in 293 cells. Expressed proteins were used for in vivo apoptosis studies. (0572)

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J. Biol. Chem. 272, 30822-30827. The Insulin-like Growth Factor-I Receptor Is Required for EWS/FLI-1 Transformation of Fibroblasts 1997

Toretsky, J.A., Kalebic, T., Blakesley, V., LeRoith, D., Helman, L.J.

Notes: The pCI-neo Mammalian Expression Vector was used to express the 1400bp EWS/FLI-1 protein in fibroblast W- and R- cells which are cells derived from transgenic mice either expressing the wildtype IGF-1 receptor (W) or a knockout of the receptor (R). (0278)

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J. Biol. Chem. 272, 12076-12082. Transfection analysis of expression of mRNA isoforms encoding the nuclear autoantigen La/SS-B. 1997

Grolz, D. , Laubinger, J. , Wilmer, F. , Troster, H. , Bachmann, M.

Notes: Both pCI and pCI-neo Mammalian Expression Vectors were used to express proteins in Mouse LTA and NIH3T3 cells and human HeLa cells. The authors report, 'In general we observed that even after optimization of the transfection conditions the expression level of the pCI-neo constructs was only 25% compared with pCI constructs.' (1122)

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EMBO J. 16, 3935-3943. ZEB, a vertebrate homolog of Drosophila Zfh-1, is a negative regulator of muscle differentiation. 1997

Postigo, A.A., Dean, D.C.

Notes: Both ZEB and the DNA-binding domain of ZEB were subcloned into Promega's pCI-neo Mammalian Expression Vector just behind a FLAG epitope. After cotransfection with reporter vectors, the effect of the expressed protein was assessed in 10T1/2 fibroblasts and C2C12 cells. The two protein inserts were subcloned into a T3 RNA polymerase binding site bearing plasmid and the flag-tagged proteins were expressed in vitro with the TNT® Coupled Reticulocyte Lysate System. (0542)

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J. Med. Chem. 39, 1339-1343. Studies on selectin blocker. 1. Structure-activity relationships of sialyl Lewis X analogs. 1996

Ohmoto, H., Nakamura, K., Inoue, T., Kondo,N., Inoue, Y., Yoshino, K., Kondo, H., Ishida, H., Kiso, M., Hasegawa, A.

Notes: The pCI-neo Mammalian Expression Vector was used to produce stable CHO cell transfectants expressing a fusion of selectin fused to the hinge and Fc regions of human IgG1 heavy chain. (0594)

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