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J. Biol. Chem. 273, 26269-26272. BEGAIN (brain-enriched guanylate kinase-associated protein), a novel neuronal PSD-95/SAP90-binding protein. 1998

Deguchi, M., Hata, Y., Takeuchi, M., Ide, B., Hirao, K., Yao, I., Irie, M., Toyoda, A., Takai, Y.

Notes: CHO cells stably transfected with the PSD-95/SAP90 protein were generated using the pCI-Neo Mammalian Expression Vector. These stably transfected cells or standard CHO cells were transiently transfected with various eukaryotic expression constructs including some in the pCI-neo Vector. Transient transfection of the CHO cells was performed with the TransFast™ Reagent. (1267)

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J. Biol. Chem. 273, 9158–9167. Caspase cleavage of gene products associated with triplet expansion disorders generates truncated fragments containing the polyglutamine tract. 1998

Wellington, C.L., Ellerby, L.M., Hackam, A.S., Margolis, R.L., Trifiro, M.A., Singaraja, R., McCutcheon, K., Salvesen, G.S., Propp, S.S., Bromm, M., Rowland, K.J., Zhang, T., Rasper, D., Roy, S., Thornberry, N., Pinsky, L., Kakizuka, A., Ross, C.A., Nicholson, D.W., Bredesen, D.E., and Hayden, M.R.

Notes: The TNT® Coupled Wheat Germ Extract and the TNT® Coupled Rabbit Reticulocyte Lysate Systems were used to in vitro express and 35S-methionine label various Huntington protein mutants and polyglutamine-containing proteins. These proteins included a full-length 210KDa Huntington, a 210KDa Atrophin-1, and a 140KDa Androgen Receptor protein. The expressed proteins were incubated with apoptotic extracts or purified caspases to detect cleavage by caspases. Mutated Huntington constructs were cloned into the pCI-neo Vector.  Data from autoradiographs were quantitated by laser band densitometry. (3019)

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J. Biol. Chem. 273, 6525-6532. Characterization of a cytosolic heat-shock protein-caveolin chaperone complex: Involvement in cholesterol trafficking 1998

Uittenbogaard, A., Ying.Y.-S., Smart, E.J.

Notes: The pCI-neo Mammalian Expression Vector was used to express the 22kDa caveolin I protein in L1210-JF lymphoid cells, which lack the caveolin protein. The expressed protein formed a chaperone complex just like NIH3T3 cells, which naturally express the protein. (0251)

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J. Biol. Chem. 273, 20721-20727. Cloning and characterization of the Hakata antigen, a member of the ficolin/opsonin p35 lectin family. 1998

Sugimoto, R., Yae, Y., Akaiwa, M., Kitajima, S., Shibata, Y., Sato, H., Hirata, J., Okochi, K., Izuhara, K., Hamasaki, N.

Notes: The cDNA for the 35kDa Hakata antigen was subcloned into the pCI-neo Mammalian Expression Vector and transiently expressed in the PLC human liver cell line. Transfection was performed with Tfx™-50 Reagent. (0312)

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J. Clin. Invest. 101, 1320-1325. Correction of renal tubular acidosis in carbonic anhydrase II-deficient mice with gene therapy. 1998

Lai, L.W., Chan, D.M., Erickson, R.P., Hsu, S.J., Lien, Y.H.

Notes: Cloned carbonic anhydrase II gene into the pCI-neo Mammalian Expression System and used in gene therapy to correct deficiency in renal tubules of carbonic anhydrase-deficient mice. (0872)

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J. Biol. Chem. 273, 35201-35207. Degradation of proto-oncoprotein c-Rel by the ubiquitin-proteasome pathway. 1998

Chen, E. , Hrdlickova, R. , Nehyba, J. , Longo, D. L. , Bose, H. R. Jr, Li, C. C.

Notes: C-terminally truncated versions of c-rel were sythesized using the TNT® Coupled Reticulocyte Lysate System. Proteins were purified away from unincorporated methionine using Centricon-30 ultrafiltration membranes. The pCI-neo Mammalian Expression Vector was used to express chicken c-rel in NIH3T3 and COS cells. (1322)

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Immunity 9, 871-880. Direct suppression of Stat1 function during adenoviral infection. 1998

Look, D.C., Roswit, W.T., Frick, A.G., Gris Alevy, Y., Dickhaus, D.M., Walter, M.J., Holtzman, M.J.

Notes: Used Dual-Luciferase® Reporter Assay System with a 400:1 transfection ratio of the firefly luciferase plasmid to pRL-SV40 Vector. Also used the pCI-neo Mammalian Expression Vector to make several E1A expression plasmids. (0743)

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J. Lipid Res. 39, 1731-1739. Human geranylgeranyl diphsphatae synthase: Isolation of the cDNA, chromosomal mapping and tissue expression 1998

Ericsson, J., Greene, J.M., Carter, K.C., Shell, B.K., Duan, D.R., Florence, C., Edwards, P.A.

Notes: The 903 nucleotide geranylgeranyl diphosphate synthase cDNA was cloned into the pCI-neo Mammalian Expression Vector and transiently expressed in CHO cells. (1223)

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Diabetes 47, 1243-1252. Insulin receptor signaling in the beta-cell influences insulin gene expression and insulin content: evidence for autocrine beta-cell regulation. 1998

Xu, G. G. , Rothenberg, P. L.

Notes: This paper describes the use of  the pCI-neo Mammmalian Expression Vector to overexpress human insulin receptor in βTC6-F7 β cells. (0123)

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J. Biol. Chem. 273, 16778-16781. Localization of the functional domains of human tissue inhibitor of metalloproteinases-3 and the effects of a Sorsby's fundous dystrophy mutation. 1998

Langton, K.P., Barker, M.D., McKie, N.

Notes: The pCI-neo Mammalian Expression Vector was used to express various mutant and wildtype tissue inhibitor of metalloproteinases (TIMP)-2 and TIMP-3 molecules in COS-7 cells. The 24-27kDa proteins were used to assess the function of the c-terminal and n-terminal domains for their metalloproteinase inhibitory functions and extracellular matrix binding functions. (0837)

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J. Biol. Chem. 273, 33561-33565. Protein phosphatase X interacts with c-Rel and stimulates c-Rel/nuclear factor κB activity. 1998

Hu, M.C., Tang-Oxley, Q., Qiu, W.R. , Wang, Y.P., Mihindukulasuriya, K.A., Afshar, R., Tan, T.H.

Notes: The authors used pCI-neo Mammalian Expression Vector to express HA-tagged PPX in 293T cells. (1021)

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Oncogene 16, 1217-1222. Telomerase activity is restored in human cells by ectopic expression of hTERT (hEST2), the catalytic subunit of telomerase 1998

Counter, C.M., Meyerson, M., Eaton, E.N., Ellisen, L.W., Caddle, S.D., Haber, D.A., Weinberg, R.A.

Notes: The TERT protein with a hemagglutinin tag was expressed in 293 cells and GM847 SV40-transformed human fibroblasts using the pCI-Neo Mammalian Expression Vector. (1282)

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J. Biol. Chem. 273, 16289-16296. The β subunit of the high conductance calcium-activated potassium channel: Identification of residues involved in charybdotoxin binding. 1998

Hanner, M., Vianna-Jorge, R., Kamassah, A., Schmalhofer, W.A., Knaus, H.-G., Kaczorowski, G.J., Garcia, M.L.

Notes: The pCI-neo Mammalian Expression Vector was used to express the α subunit (>104kDa) of the high conductance Ca2+-activate K+ channel as well as various mutants of the β subunit (~30kDa) in COS-1 cells and TsA-201 cells (HEK 293 cells transformed with the SV40 T antigen). (1062)

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Science 279, 102-105. Ultraviolet-induced cell death blocked by a selenoprotein from a human dermatotropic poxvirus 1998

Shisler, J. L., Senkevich, T. G., Berry, M. J., Moss, B.

Notes: The authors used Promega's pCI Mammalian Expression Vector to create several expression constructs. (0398)

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Cell 90(2), 303-311. A human endogenous retroviral superantigen as candidate autoimmune gene in type I diabetes. 1997

Conrad, B., Weissmahr, R.N., Böni, J., Arcari, R., Schüpbach, J. and Mach, B.

Notes: The inhibitor was used to protect RNA during RT-PCR. (1671)

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Cell 90, 303-313. A human endogenous retroviral superantigen as candidate. Autoimmune gene in type I diabetes 1997

Conrad, B., Weissmahr, R.N., Böni, J., Arcari, R., Schüpbach, J. and Mach, B.

Notes: The pCI-Neo Mammalian Expression Vector was used to express a truncated env protein in THP-1 cells. (2014)

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J. Cell Sci. 110, 1759-1765. A specific domain in α-catenin mediates binding to β-catenin or plakoglobin. 1997

Huber, O., Krohn, M., Kemler, R.

Notes: The cDNA for α-catenin was subcloned into the pCI-neo Mammalian Expression Vector, and a synthetic 6XHis-Tag was added to the cDNA. A deletion mutation of α-catenin was also subcloned into the vector. The His-tag was used to bring down complexes of the proteins, which were then probed on Western blots for the individual proteins. (1031)

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J. Biol. Chem. 272, 7765-7769.. Alterations in receptor activation and divalent cation activation of agonist binding by deletion of intracellular domains of the glucagon receptor. 1997

Chicchi, G. G. , Graziano, M. P. , Koch, G. , Hey, P. , Sullivan, K. , Vicario, P. P. , Cascieri, M. A.

Notes: The pCI-Neo Mammalian Expression Vector was used to express wildtype and mutant glucagon receptors either transiently (COS cells) or stably (CHO cells). The expressed protein was used for numerous assays of receptor function and receptor:ligand interactions. (1338)

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Neuron 18, 857-863. Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy. 1997

Lang, T., Wacker, I., Steyer, J., Kaether, C. Wunderlich, I., Soldati, T., Gerdes, H.H. and Almers, W.

Notes: The vector is used to express a human Neuropeptide Y-GFP fusion protein in PC12 cells. (1583)

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J. Biol. Chem. 272, 27218-27223. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. 1997

Karlsson, M., Contreras, J.A., Hellman, U., Tornqvist, H., Holm, C.

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

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J. Biol. Chem. 272, 6324-6331. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. 1997

Naslavsky, N., Stein, R., Yanai, A., Friedlander, G., Taraboulos, A.

Notes: The pCI-Neo Mammalian Expression Vector was used to express the prion PrP in mouse N2a cells. (0647)

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Mol. Pharmacol. 52, 292-299. CholecystokininB receptor from human Jurkat lymphoblastic T cells is involved in activator protein-1-responsive gene activation 1997

Oiry, C. , Gagne, D. , Cottin, E. , Bernad, N. , Galleyrand, J. C. , Berge, G. , Lignon, M. F. , Eldin, P. , Le Cunff, M. , Leger, J. , Clerc, P. , Fourmy, D. , Martinez, J.

Notes: The cholecystokinin (CCKb) receptor was expressed in COS-7 cells using the pCI-neo Mammalian Expression Vector. The cells were used for cell surface ligand binding studies. The Transfectam® Reagent was used to transiently transfect Jurkat T cells. Many details of the transfection are provided. (0597)

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J. Biol. Chem. 272, 31738-31746. Cig30, a mouse member of a novel membrane protein gene family, is involved in the recruitment of brown adipose tissue 1997

Tvrdik, P., Asadi, A., Kozak, L.P., Nedergaard, J., Cannon, B., Jacobsson, A.

Notes: The pCI-neo Mammalian Expression Vector was used to construct a chimeric vector with the neo resistance gene downstream of an internal ribosome entry site, so that the neo gene was transcribed with the Cig30 cDNA and both protein controlled by the CMV promoter. The construct was used to make stable transfectants of the HIB-1B hibernoma cells. Cell lines were analyzed by Northern blotting. The protein was also analyzed in vitro with the Rabbit Reticulocyte Lysate and Canine Pancreatic Microsomal Membranes (CMMs). (0246)

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J. Biol. Chem. 272, 27577-27581. Complex subunit assembly of neuronal voltage-gated K+ channels. Basis for high-affinity toxin interactions and pharmacology. 1997

Koch, R.O., Wanner, S.G., Koschak, A., Hanner, M., Schwarzer, C., Kaczorowski, G.J., Slaughter, R.S., Garcia, M.L., Knaus, H.G.

Notes: The Kv1.1 and Kv1.2 cDNAs were each cloned into the pCI-neo Mammalian Expression Vector. The two ~80kDa proteins were transiently expressed in COS-1 cells and assayed for charybdotoxin and α-dendrotoxin binding as well as recognition by antisera. (0914)

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J. Biol. Chem. 272, 14532-14541. Cytotoxicity and apoptosis produced by arachidonic acid in Hep G2 cells overexpressing human cytochrome P4502E1. 1997

Chen, Q. , Galleano, M. , Cederbaum, A. I.

Notes: Both sense and antisense bcl-2 and cytochrome P4502E1 cDNAs were stably expressed in a HepG2 derived cell line using the pCI-Neo Mammalian Expression Vector. Cytotoxicity of arachidonic acid to transfected and control cells was assessed using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (1328)

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