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J. Virol. 76, 12032-12043. Baculorus lef-12 is not required for viral replication. 2002

Guarino, L.A. Mistretta, T.-A. and Dong, W.

Notes: RT-PCR was used to confirm the expression of the lef-12 gene at various times post-infection. The RT step was performed using ImProm-II™ Reverse Transcriptase in a 20µl reaction volume. One microliter of the RT reaction was amplified in a 50µl PCR amplification reaction using the PCR Master Mix. (2628)

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