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Promega Corporation

Automated Methods

Sample Type Instrument
(click for method downloads)
Sample Volume (or amount) Method Time (minutes) Kit Chemistry Type
Blood Beckman Coulter Biomek® FX 200µl anticoagulated whole human blood 105 MagneSil® Genomic, Max Yield Magnetic Resin-Based
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Blood Tecan Freedom EVO® 1-10ml whole human blood 32 samples in 4 hours ReliaPrep™ Large Volume HT gDNA System Magnetic Resin-Based
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  • TB: TM341
  • EP: N/A
  • Output: ≥300µg human genomic DNA from 10ml sample; yield is variable based on WBC count and sample volume processed.
  • Automation Company Website: Tecan
Blood Beckman Coulter Biomek® FX ≤350µl anticoagulated whole human blood 150 ReliaPrep™ 96 gDNA Miniprep HT System Magnetic Resin-Based
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  • TB: TM368
  • EP: N/A
  • Output: Up to 12µg human genomic DNA
  • Automation Company Website: Beckman Coulter
Cultured Cells Beckman Coulter Biomek® 2000 <1x105 cultured cells 60 MagneSil® RNA Magnetic Resin-Based
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Cultured Cells Beckman Coulter Biomek® 3000 <1x105 cultured cells 60 MagneSil® RNA Magnetic Resin-Based
Cultured Cells Beckman Coulter Biomek® FX <1x103 cultured cells 100 MagneSil® RNA, 384 Well Magnetic Resin-Based
Cultured Cells Beckman Coulter Biomek® FX <1x105 cultured cells 30 MagneSil® RNA, 96 Well Magnetic Resin-Based
Cultured Cells Beckman Coulter Biomek® 2000 ≤5x106 cultured cells 45 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Cultured Cells Beckman Coulter Biomek® 3000 ≤5x106 cultured cells 35 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Cultured Cells Perkin Elmer Janus® ≤5x106 cultured cells 45 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Cultured Cells Beckman Coulter Biomek® FX ≤5x106 cultured cells 55 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Cultured Cells Beckman Coulter Biomek® 2000 ≤5x106 cultured cells 70 SV 96 Total RNA Vacuum Filtration-Based
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Cultured Cells Beckman Coulter Biomek® 3000 ≤5x106 cultured cells 70 SV 96 Total RNA Vacuum Filtration-Based
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Cultured Cells Beckman Coulter Biomek® FX ≤5x106 cultured cells 60 SV 96 Total RNA Vacuum Filtration-Based
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Cultured Cells Eppendorf epMotion® 5075 VAC ≤5x106 cultured cells 90 SV 96 Total RNA Vacuum Filtration-Based
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Cultured Cells Perkin Elmer Janus® ≤5x106 cultured cells 70 SV 96 Total RNA Vacuum Filtration-Based
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Blood Beckman Coulter Biomek® 3000 <20µl whole human blood 60 MagneSil® RNA Magnetic Resin-Based
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Blood Beckman Coulter Biomek® FX 60µl human whole blood 60 MagneSil® ONE Fixed Yield Magnetic Resin-Based
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PCR Reaction Beckman Coulter Biomek® 2000 ≤100µl PCR Reaction 40 Wizard® SV 96 PCR Clean-Up Vacuum Filtration-Based
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PCR Reaction Eppendorf epMotion® 5075 VAC ≤100µl PCR Reaction 50 Wizard® SV 96 PCR Clean-Up Vacuum Filtration-Based
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PCR Reaction Perkin Elmer Janus® ≤100µl PCR Reaction 40 Wizard® SV 96 PCR Clean-Up Vacuum Filtration-Based
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PCR Reaction Beckman Coulter Biomek® FX ≤100µl PCR Reaction 20 Wizard® SV 96 PCR Clean-Up Vacuum Filtration-Based
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Plant Beckman Coulter Biomek® 2000 8mm leaf disc or 1-5 seeds 40 MagneSil® Plant Magnetic Resin-Based
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Plant Eppendorf epMotion® 5075 LH 8mm leaf disc or 1-5 seeds 60 MagneSil® Plant Magnetic Resin-Based
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Plant Beckman Coulter Biomek® FX 8mm leaf disc or 1-5 seeds 25 MagneSil® Plant Magnetic Resin-Based
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Plant Beckman Coulter Biomek® 3000 8mm leaf disc or 1-5 seeds 40 MagneSil® Plant Magnetic Resin-Based
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Plant Beckman Coulter Biomek® FX 1-2 leaf punches (6-8mm) or 1-2 seeds 40 MagneSil® Plant, Fixed Yield Magnetic Resin-Based
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Plant Beckman Coulter Biomek® FX 1-10 leaf punches (6-8mm) or 1-5 seeds 50 MagneSil® Plant, Inc Yield Magnetic Resin-Based
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Plasmid Beckman Coulter Biomek® FX Bacterial culture ≤6 OD600 45 MagneSil® Plasmid Magnetic Resin-Based
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Plasmid Beckman Coulter Biomek® 2000 Bacterial culture ≤6 OD600 75 MagneSil® Plasmid Magnetic Resin-Based
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Plasmid Tecan Freedom EVO® Bacterial culture ≤6 OD600 75 MagneSil® Plasmid Magnetic Resin-Based
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Plasmid Beckman Coulter Biomek® NX bacterial culture ≤6 OD600 75 MagneSil® Plasmid Magnetic Resin-Based
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Plasmid Beckman Coulter Biomek® FX bacterial culture ≤6 OD600 45 MagneSil® Plasmid Tfx Magnetic Resin-Based
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Plasmid Beckman Coulter Biomek® 2000 bacterial culture ≤6 OD600 75 MagneSil® Plasmid Tfx Magnetic Resin-Based
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Plasmid Beckman Coulter Biomek® FX bacterial culture ≤4 OD600 60 Wizard® SV 96 Plasmid Vacuum Filtration-Based
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Plasmid Beckman Coulter Biomek® 2000 bacterial culture ≤4 OD600 80 Wizard® SV 96 Plasmid Vacuum Filtration-Based
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Plasmid Eppendorf epMotion® 5075 VAC bacterial culture ≤4 OD600 90 Wizard® SV 96 Plasmid Vacuum Filtration-Based
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Plasmid Perkin Elmer Janus® bacterial culture ≤4 OD600 80 Wizard® SV 96 Plasmid Vacuum Filtration-Based
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Sanger Sequencing Beckman Coulter Biomek® 2000 20µl sequencing reaction 45 MagneSil® Sequencing Magnetic Resin-Based
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  • TB: TB287
  • EP: EP021
  • Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp
  • Automation Company Website: Beckman Coulter
Sanger Sequencing Tecan Freedom EVO® 20µl sequencing reaction 45 MagneSil® Sequencing Magnetic Resin-Based
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  • TB: TB287
  • EP: EP021
  • Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp
  • Automation Company Website: Tecan
Sanger Sequencing Beckman Coulter Biomek® NX 20µl sequencing reaction 45 MagneSil® Sequencing Magnetic Resin-Based
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  • TB: TB287
  • EP: EP021
  • Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp
  • Automation Company Website: Beckman Coulter
Sanger Sequencing Beckman Coulter Biomek® FX 20µl sequencing reaction 25 MagneSil® Sequencing Magnetic Resin-Based
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  • TB: TB287
  • EP: EP021
  • Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp
  • Automation Company Website: Beckman Coulter
Sanger Sequencing Beckman Coulter Biomek® FX 10µl sequencing reaction 45 MagneSil® Sequencing, 384 well Magnetic Resin-Based
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Tissue Perkin Elmer Janus® ≤5x106 cultured cells 70 SV 96 Total RNA Vacuum Filtration-Based
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Saliva Beckman Coulter Biomek® FX ≤700µl Oragene saliva 150 ReliaPrep™ 96 gDNA Miniprep HT System Magnetic Resin-Based
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  • TB: TM368
  • EP: N/A
  • Output: Up to 12µg human genomic DNA
  • Automation Company Website: Beckman Coulter
Tissue Beckman Coulter Biomek® 2000 ≤20mg of tissue lysate; ≤1.2cm of mouse tail 50 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Tissue Beckman Coulter Biomek® FX ≤20mg of tissue lysate; ≤1.2cm of mouse tail 40 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Tissue Beckman Coulter Biomek® 3000 ≤20mg of tissue lysate; ≤1.2cm of mouse tail 50 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Tissue Eppendorf epMotion® 5075 VAC ≤20mg of tissue lysate; ≤1.2cm of mouse tail 60 Wizard® SV 96 Genomic Vacuum Filtration-Based
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Tissue Beckman Coulter Biomek® 2000 ≤30mg of tissue lysate 70 SV 96 Total RNA Vacuum Filtration-Based
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Tissue Beckman Coulter Biomek® 3000 ≤30mg of tissue lysate 70 SV 96 Total RNA Vacuum Filtration-Based
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Tissue Perkin Elmer Janus® ≤30mg of tissue lysate 70 SV 96 Total RNA Vacuum Filtration-Based
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Tissue Beckman Coulter Biomek® FX ≤30mg of tissue lysate 60 SV 96 Total RNA Vacuum Filtration-Based
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Tissue Eppendorf epMotion® 5075 VAC ≤30mg of tissue lysate 90 SV 96 Total RNA Vacuum Filtration-Based
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Tissue Beckman Coulter Biomek® 2000 <2mg tissue lysate per well 60 MagneSil® RNA Magnetic Resin-Based
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Tissue Beckman Coulter Biomek® 3000 <2mg tissue lysate per well 60 MagneSil® RNA Magnetic Resin-Based
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Tissue Beckman Coulter Biomek® FX <2mg tissue lysate per well 30 MagneSil® RNA, 96 Well Magnetic Resin-Based
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Blood Beckman Coulter Biomek® 2000 <20µl whole human blood 60 MagneSil® RNA Magnetic Resin-Based
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Blood Beckman Coulter Biomek® FX <5µl whole human blood per well 100 MagneSil® RNA, 384 Well Magnetic Resin-Based
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Blood Beckman Coulter Biomek® FX <20µl whole human blood 30 MagneSil® RNA, 96 Well Magnetic Resin-Based
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