Development of PowerSeq™ Systems for Forensic Identification Using Next Generation Sequencing

Part # PS252

Abstract

Lotte Downey, Jaynish Patel, Spencer Hermanson, Leta Steffen, Cynthia Sprecher, Robert S. McLaren, and Douglas R. Storts. Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, U.S.A.

STR-based systems validated for forensics generate fluorescently-labeled amplicons that are separated by capillary electrophoresis (CE). Allele calling is based on the size and fluorescent label of the amplified alleles. Next generation sequencing (NGS) based methods detect the actual sequence.

The ability to assay more loci can enable generation of autosomal STR, Y-STR, single nucleotide polymorphism, and mitochondrial DNA (mtDNA) data from the same sample in a single experiment. Smaller amplicons allows for more data generation from degraded DNA samples.

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