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Promega Corporation

MAO-Glo™ Assay: Fast and Easy Luminescent Assay to Measure Monoamine Oxidases...

MAO-Glo™ Assay: Fast and Easy Luminescent Assay to Measure Monoamine Oxidases Scientific Poster



M.P. Valley1, W. Zhou2, E.M. Hawkins1, J. Shultz1, T. Good2, D. Good2, K.V. Wood1
1Promega Corporation, 2800 Woods Hollow Rd. Madison, WI 53711, 2Promega Biosciences Inc, 277 Granada Dr., San Luis Obispo, CA 93401

The MAO-Glo™ Assay, a luminescent method for sensitive quantitation of monoamine oxidase (MAO) activity, enables rapid assessment of potential inhibition caused by new chemical entities. The homogeneous assay format is compatible with laboratory automation, or can be performed manually (e.g., 96 samples in under 90 minutes). The assay is suitable for both MAO A and MAO B, and data generated for known MAO inhibitors yielded Ki and IC50 values consistent with published literature. The signal-to-noise ratio is >1000 and Z' values ~ 0.95. Data concerning compounds from the LOPAC library known as MAO A inhibitors or substrates were extracted from the screen.

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  • Part# PS004
  • Printed in USA.

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