We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Improvements to Luciferase Reporter Assays for Nuclear Receptor Function Scientific Poster



Aileen Paguio, Pete Stecha, Frank Fan and Keith Wood
Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711

Luciferase reporter assays are frequently used to study nuclear receptor function because of their wide dynamic range, low endogenous activity and ease of use. Recent improvements to pGL4 Luciferase Reporter vectors have further enhanced their utility for research and drug discovery. We used an assay containing the ligand binding domain (LBD) of a nuclear receptor fused to the Gal4 DNA binding domain (DBD) on one vector and multiple Gal4 upstream activator sequences (UAS) upstream of luciferase reporter on another vector to monitor ligand binding and transactivation. We showed that codon optimization of luciferase reporter genes increased expression  levels. Incorporation of protein destabilizing sequences into luciferase leads to a larger assay dynamic range up to several hundred fold. The optimum number of UAS to generate the best response was also evaluated.

Document Icon Download the poster PDF (71kb)

  • Part# PS065
  • Printed in USA.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.