We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

A Homogeneous Bioluminescent Assay for High-Throughput Antimicrobial Screening...

A Homogeneous Bioluminescent Assay for High-Throughput Antimicrobial Screening Scientific Poster



Frank Fan, Braeden Butler, Terry Riss and Keith Wood
Promega Corporation, 2800 Woods Hollow Road, Madison WI 53711

ATP-based detection of microbial cells represents a key application of luciferase/luciferin bioluminescence assay. Conventional methods require two steps: application of a lysis reagent to release microbial ATP, followed by a detection reagent to elicit bioluminescence. We have developed an assay that combines the lytic reagent with luciferase/luciferin, thus allowing sensitive
detection of microbial cells in a single-step. The assay system utilizes a thermostable luciferase to enable extraction of ATP from bacterial cells and to support a stable “glow-type” luminescent signal. In addition, we developed a formulation to achieve rapid and efficient extraction of ATP from a variety of microbial cells. The combination of these two essential elements enabled the design of a homogeneous single-reagent system (BacTiter-Glo™ Reagent) for performing ATP assays on cultured cells.

Document Icon Download the poster PDF (80kb)

  • Part# PS010
  • Printed in USA.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.