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FFPE Quality: Impact on Downstream DNA Performance in Molecular Diagnostic Assays Poster

Part # PS232

Abstract

Jonathan M. Eibl, Julie A. Toplin, Chad D. Galderisi, Cindy S. Spittle, MolecularMD Corp., Portland, OR

Personalized medicine for oncology patients is driven by mutation profiling of tumors. Improved robust DNA extraction methods that produce high quality FFPE DNA template suitable for complex molecular diagnostic testing are needed. In addition to DNA fragmentation and protein‐DNA cross linking, some FFPE tissues contain inhibitors and/or quenching substances (e.g., melanin) that adversely affect downstream performance in PCR and sequencing assays. In this study, we provide a side‐by‐side evaluation of two DNA isolation methods from FFPE tissue of various tumor types: a manual, column‐based method (MO BIO) and the Maxwell CSC automated platform (Maxwell).

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