Fast Accurate Fluorescence Quantitation of FFPE Samples from Maxwell® 16 using QuantiFluor® ONE dsDNA Dye and the Quantus™ Fluorometer

Part # PS227

Abstract

Mark Denhart and Michael Bjerke
Promega Corporation 2800 Woods Hollow Road, Madison, WI 53711

Nucleic Acid quantitation improves success in a variety of downstream assays such as PCR, Cloning, Next Gen Sequence (NGS), and DNA transfections. Some sample types, such as formalin-fixed paraffin-embedded (FFPE) tissues, contain low levels of nucleic acid due to their harsh sample preservation methods. Absorbance quantitation methods, such as NanoDrop®, lack adequate detection sensitivity as well as target-specificity for low-level sample types Fluorescence methods provide 200 times more sensitivity than NanoDrop® Spectrophotometry, allowing researchers to accurately quantitate these samples.

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