Correlation of Mutations Detected in Liquid and Tissue Biopsies

Part # PS248

Abstract

Douglas Horejsh1, Douglas H. White1, Molly A. Accola2, William M. Rehrauer2, Jeffrey Franz1, Herly Karlen1 and Marjeta Urh1
1Promega Corporation, Madison, Wisconsin 2Molecular Diagnostics Laboratory, University of Wisconsin Hospital and Clinics, Madison, Wisconsin

Circulating cell-free DNA (ccfDNA) in plasma can be used to detect biomarkers that show great promise for diagnosis and monitoring of cancer, giving rise to the possibility of liquid biopsies that obviate the need for invasive tissue collection. Here, we describe a method for purifying nucleic acids based on novel surface and binding chemistries. The combination of these two approaches allows for increased binding of fragmented DNA. The method can be partially automated to ensure highly reproducible results.


Printed in USA.