Automated RNA Purification from Salmonid Organs Using a Novel Paramagnetic Particle Technology Poster

Part # PS238

Abstract

Kevin Kershner1, Doug Wieczorek PhD1, Laurence Delauriere PhD2, Eric B. Vincent PhD1, Trista Schagat1 PhD
1Promega Corporation, 2800 Woods Hollow, Madison WI, USA 53711; 2Promega Europe Training and Applications Laboratory (PETAL), 24 Chemin des Verrières, Charbonnières-les-Bains, France 69260

RNA viruses, including viral hemorrhagic septicemia virus, Salmonid alphaviruses (SAV) and infectious salmon anemia virus (ISAV), pose a significant threat to Atlantic salmon populations. Molecular techniques offer the means of monitoring for these viruses in fish farms as well as wild salmon populations. To facilitate rapid, reliable testing, we explore the use of magnetic particle-based purification technologies for automated extraction of RNA from salmon and trout organs.

Printed in USA.