We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Automated Miniaturization of Promegas Kinase-Glo Luminescent Kinase Assay Scientific...

Automated Miniaturization of Promegas Kinase-Glo Luminescent Kinase Assay Scientific Poster



Brad Larson, B.A.1, Tracy Worzella, M.S.1, Michael Bjerke, M.S.1, Aoife Gallagher, Ph.D.2 and Eric Matthews, M.S.3
1Promega Corporation, Madison, WI, 2Deerac Fluidics, Dublin, Ireland, 3BMG Labtech

Protein kinases play a vital role in cellular activity, ranging from cell proliferation to cell death. Their substrates encompass a wide range of chemical structures such as proteins, peptides, sugars, lipids, etc. They are also implicated in many diseases, including cancer, inflammatory responses, and Alzheimer’s disease. This makes them ideal targets for high-throughput screening of potential drug targets. Because of increased demands on screening facilities, the ability to miniaturize assays has become imperative. We have developed automated Kinase-Glo® methods in low-volume 384- and 1536-well plate formats using the Deerac Fluidics Equator™ NS 808 nanoliter-scale noncontact liquid handler. Luminescent kinase activity was measured with the BMG LABTECH PHERAstar reader. The Kinase-Glo® Assay is a homogeneous method for measuring kinase activity from virtually any kinase/substrate combination by quantifying ATP remaining in solution following a kinase reaction. Excellent Z′-factor scores and accurate IC50 values in reaction volumes as low as 2μl, coupled with the extended luminescent half-life of this reagent chemistry, make this assay ideal for all high-throughput applications.

Document Icon Download the poster PDF (449kb)

  • Part# PS026
  • Printed in USA.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.