Recently we developed a novel product, the MagZ™ Protein Purification System, for the purification of expressed his-tagged protein from rabbit reticulocyte lysate. This protein purification system has a unique advantage over nickel-based immobilized metal ion affinity chromatography (IMAC) systems in that it specifically purifies His-tagged proteins from rabbit reticulocyte lysate without co-purifying hemoglobin. This article demonstrates that MagZ™ Binding Particles can be used to develop in vitro His-tag pull-down assays. By using a His-tagged Id protein attached to MagZ™ Particles, we show that we can detect the interaction with MyoD.
Promega Notes 88, 13–15.
Becky Godat and Tonny Johnson