Technically Speaking: RNA Interference
RNA interference (RNAi) is a rapidly emerging and powerful technique used to investigate gene function by degrading a specific mRNA target in a cell or organism and thus knocking out or knocking down the level of the encoded protein. This specific mRNA degradation is mediated by complementary double-stranded RNA. This article reviews RNAi and its relationship to other RNA-mediated mechanisms generally referred to as RNA silencing. This review also addresses the involvement of short interfering RNAs (siRNAs), microRNAs (miRNAs), small temoral RNAs (stRNAs), and short hairpin RNAs (shRNAs) in various RNA silencing mechanisms.
Promega Notes 83, 33–36.
Publication Date: 2003