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Promega Corporation

Introducing the Kinase-Glo® Luminescent Kinase Assay

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Abstract

The Kinase-Glo™ Luminescent Kinase Assay is a homogeneous method for measuring kinase activity by quantifying a decrease in ATP levels following kinase activity. The assay can work with virtually any kinase and substrate combination. The assay is performed in a single well of a standard multiwell plate by adding an equal volume of Kinase-Glo™ Reagent and reading luminescence. The assay produces excellent Z´ values, easily detects known kinase inhibitors, and provides accurate IC50 values.

Promega Notes 83, 14–17.

Richard Somberg, Becky Pferdihert and Kevin Kupcho

Promega Corporation
Publication Date: 2003

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