The Kinase-Glo™ Luminescent Kinase Assay is a homogeneous method for measuring kinase activity by quantifying a decrease in ATP levels following kinase activity. The assay can work with virtually any kinase and substrate combination. The assay is performed in a single well of a standard multiwell plate by adding an equal volume of Kinase-Glo™ Reagent and reading luminescence. The assay produces excellent Z´ values, easily detects known kinase inhibitors, and provides accurate IC50 values.
Promega Notes 83, 14–17.
Richard Somberg, Becky Pferdihert and Kevin Kupcho