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CaspACE™ FITC-VAD-FMK In Situ Marker as a Probe for Flow Cytometry Detection of...

CaspACE™ FITC-VAD-FMK In Situ Marker as a Probe for Flow Cytometry Detection of Apoptotic Cells
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Abstract

CaspACE™ FITC-VAD-FMK In Situ Marker, a fluorescent derivative of VAD-FMK, was used to label apoptotic cells in a population of leukemia cells for analysis by flow cytometry. This marker was able to discriminate between early and late Fas-driven apoptosis in Jurkat T cells. CaspACE™ FITC-VAD-FMK was thus found to be a reliable marker for labeling apoptotic cells prior to flow cytometry.

Promega Notes 76, 10–13.

Francis Belloc, Olivier Garnier, Catherine Boyer and Francis Lacombe

Laboratoire d'Hématologie, Hôpital du Haut Lévêque, F-33604 Pessac, France
Publication Date: 2000

Download Article (321 KB)

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