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A HTS System for Screening Antiviral Compounds Using the CellTiter® 96 AQueous One...

A HTS System for Screening Antiviral Compounds Using the CellTiter® 96 AQueous One Solution Assay
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Abstract

The CellTiter 96® AQueous One Solution Assay features the MTS tetrazolium compound, which is converted by viable cells into an intensely colored formazan product that is directly soluble in tissue culture media. The reagents in the CellTiter 96® AQueous One Solution System enable design of homogeneous high-throughput screening assays to monitor cell viability as an endpoint. In this article, a virus-induced cytopathic effect (CPE)-inhibition assay using Promega's CellTiter® 96 AQueous One Solution Assay is used to evaluate compounds for antivial activity against a variety of viruses.

Promega Notes 75, 13–16.

Thomas Fletcher, III1, Roger Ptak1, Stacy Bartram1, Susan Halliday1, Robert Buckheit, Jr.1, Rich Moravec2, and Terry Riss2

1Infectious Disease Research Department, Serquest, Southern Research Institute, Frederick, MD; 2Promega Corporation
Publication Date: 2000

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