TransFast™ Transfection Reagent is comprised of the synthetic cationic lipid, N,N [bis (2-hydroxyethyl)-N-methyl-N-[2,3-di(tetradecanoyloxy) propyl] ammonium iodide, and the neutral lipid, DOPE. The TransFast™ Reagent is supplied as a dried lipid film that forms multilamellar vesicles upon hydration with water. The experiments described in this article address some commonly asked questions about the protocol for use of this reagent.
Promega Notes 71, 18.
Jennifer Oler and Elaine Schenborn