Promega has developed a new luciferase reporter vector for
single- or dual-reporter gene measurements. Our experiments were performed in order to inhibit TNFα-induced nuclear transcription factor kappa B
(NF-κB) activity by a natural product. Results with this new vector show improved reporter expression of firefly luciferase
luminescence compared to a competitor vector. This
new vector allows more accurate and precise measurement of NF-κB inhibition by
bioactive compounds paralleled by an economically
better consumption of the firefly luciferase vector.
Cell Notes 21, 18–19.
Marc Schumacher, Mario Dicato and Marc Diederich*