The HaloTag® Interchangeable Labeling Technology provides options
for rapid, site-specific labeling of proteins in living cells and in vitro. The
technology is based on the formation of a covalent bond between HaloTag® Protein
and synthetic ligands that carry a variety of functionalities. The covalent bond
forms rapidly under physiological conditions, is highly specific and essentially
irreversible, yielding a complex that is stable even under denaturing
Cell Notes 14, 10–14.
G. Los, R. Learish, Natasha Karassina, Chad Zimprich, Mark G. McDougall, Lance P. Encell, Rachel Friedman-Ohana, Monika Wood, Gediminas Vidugiris, Kris Zimmerman, Paul Otto, Soshana Berstock, Dieter Klaubert,and Ketih V. Wood