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Promega Corporation

Assay for DPPIV Activity Using a Homogeneous Luminescent Method

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Abstract

The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent assay that measures activity of DPPIV, a serine protease that is involved in a variety of biological processes including immune regulation, signal transduction and apoptosis. In the assay, adding a single DPPIV-Glo™ Reagent results in DPPIV cleavage of the supplied substrate and generation of a "glow-type" luminescent signal that is proportional to DPPIV activity. The assay is easily amenable to high-throughput screening applications.

Cell Notes 11, 8–11.

Martha O'Brien1, Bill Daily2, Mike Schurria1, and Terry Riss1

1Promega Corporation, 2Promega Biosciences
Publication Date: 2005

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