PowerPlex® 6C Matrix Standard Technical Manual
Instructions for Use of Product(s)
DG4900
Literature # TMD046
Proper generation of a spectral calibration file is critical to evaluate multicolor systems with the Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. The PowerPlex® 6C Matrix Standard consists of DNA fragments labeled with six different fluorescent dyes (FL-6C, JOE-6C, TMR-6C, CXR-6C, TOM-6C and WEN) in one tube. The spectral calibration is performed using the J6 dye set. Once generated, the spectral calibration file is applied during sample detection to calculate the spectral overlap and separate the raw fluorescent signals into individual color signals.
The PowerPlex® 6C Matrix Standard was developed for use with the Promega 6-color PowerPlex® Systems and is compatible with the Applied Biosystems® 3500 and 3500xL Genetic Analyzers as well as Applied Biosystems® 3130 and 3130xl Genetic Analyzers with Data Collection Software Version 4.0 with the DC v4 6-Dye Module v1 License.
Printed in the USA Revised 10/15.