PowerPlex® 21 System Technical Manual

Instructions for Use of Product(s)
DC8902, DC8942
Literature # TMD034

The PowerPlex® 21 System allows co-amplification and four-color fluorescent detection of 21 loci (20 STR loci and Amelogenin), including D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX and vWA. The PowerPlex® 21 System provides all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. This manual contains a protocol for use of the PowerPlex® 21 System with the GeneAmp® PCR System 9700 thermal cycler in addition to protocols to separate amplified products and detect separated material. The system is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes: 7/14 Revision
The following changes were made to the 7/14 version of this document:
Legal disclaimers were updated, and discontinued products were removed.

Printed in USA. Revised 7/14.