PowerPlex® 21 System Technical Manual

Instructions for Use of Product(s)
DC8902, DC8942
Literature # TMD034

The PowerPlex® 21 System allows co-amplification and four-color fluorescent detection of 21 loci (20 STR loci and Amelogenin), including D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX and vWA. The PowerPlex® 21 System provides all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. This manual contains a protocol for use of the PowerPlex® 21 System with the GeneAmp® PCR System 9700 thermal cycler in addition to protocols to separate amplified products and detect separated material. The system is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes
The following changes were made to the 6/16 revision of this document:
1. The kit component CC5 Internal Lane Standard 500 (CC5 ILS 500) was replaced with the WEN Internal Lane Standard 500 (WEN ILS 500). Instructions were updated throughout the document for use of this new internal lane standard.
2. The artifacts table in Section 6.L was updated.
3. Other general updates were incorporated.
4. The document design was updated.

Printed in USA. Revised 6/16.