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Promega Corporation

PowerPlex® 18D System Technical Manual

Literature # TMD031

The PowerPlex® 18D System allows co-amplification and four-color fluorescent detection of eighteen loci, including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, FGA, D19S433 and D2S1338. The PowerPlex® 18D System provides all materials necessary to amplify STR regions of genomic DNA, including a hot-start, nonproofreading thermostable DNA polymerase. The system is optimized for analysis of common database samples, such as unwashed FTA® card punches (i.e., direct amplification). Additionally, thermal cycling time is reduced from previous PowerPlex® protocols. The PowerPlex® 18D System is compatible with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes: 5/14 Revision
The following changes were made to the 5/14 version of this document:
Legal disclaimers were updated.

Printed in USA. Revised 5/14.

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