pHAb Amine and Thiol Reactive Dyes
Instructions for Use of Product(s)
G9831, G9835, G9841, G9845
Literature # TM451
pHAb Reactive Dyes are sensor dyes that have very low fluorescence at pH > 7, and a dramatic increase in fluorescence as the pH of the solution becomes acidic. pHAb Dyes are designed specifically for labeling antibody and are available in two reactive forms suitable for antibody conjugations. pHAb Amine Reactive Dye has a succinimidyl ester group that reacts with primary amines available on the lysine amino acids on the antibodies. pHAb Thiol Reactive Dye has a maleimide group that reacts with thiols. This maleimide group is conjugated to the antibody after the cysteine disulfi de bonds in the hinge region of the antibody are reduced to thiols using a reducing agent, such as DTT or TCEP.
A key feature of pHAb Dyes is the two sulfonate groups per dye, which improve solubility in water and reduce the aggregation often seen with other non-sulfonated dyes. pHAb Dyes retain their fluorescence response to pH change, even after conjugation to antibody. Even though antibody conjugation is the key application, any protein containing primary amines on lysine amino acids or thiols on the cysteine amino acids can be conjugated with pHAb Dyes.
Summary of change
The following change was made to the 12/17 revision of this document:
References were added.
Printed in USA. Revised 12/17.