Instructions for Use of Product(s)
Literature # TM230
Promega Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus minimize background translation. Wheat Germ Extract and Rabbit Reticulocyte Lysate contain the cellular components necessary for protein synthesis (tRNA, ribosomes and initiation, elongation and termination factors).
Only the addition of exogenous amino acids (including an appropriate labeled amino acid) and mRNA are necessary to stimulate translation. Potassium Acetate is supplied as a separate component so that the translational system may be optimized for a wide range of mRNAs.
Summary of Changes
The 7/15 version of this Technical Manual was revised to:
•remove expired patent and disclaimer statements and references to discontinued products,
•align units in troubleshooting section and protocol notes, and
•update design and document formatting.
Printed in USA. Revised 7/15.
Experienced User Protocols