We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Wheat Germ Extract Technical Manual

Literature # TM230

Promega Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus minimize background translation. Wheat Germ Extract and Rabbit Reticulocyte Lysate contain the cellular components necessary for protein synthesis (tRNA, ribosomes and initiation, elongation and termination factors).
Only the addition of exogenous amino acids (including an appropriate labeled amino acid) and mRNA are necessary to stimulate translation. Potassium Acetate is supplied as a separate component so that the translational system may be optimized for a wide range of mRNAs.

Printed in USA. Revised 10/09.

 

PDF Icon Complete Protocol PDF (255kb)

PDF Icon Quick Protocol PDF (84kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.