PinPoint™ Xa Protein Purification System Technical Manual
Literature # TM028
The PinPoint™ Xa Protein Purification System is designed for the production and purification of fusion proteins that are biotinylated in vivo. Biotinylated fusion proteins are produced in E. coli and are affinity-purified using the SoftLink™ Soft Release Avidin Resin. This proprietary resin allows elution of the fusion protein under nondenaturing conditions. The PinPoint™ Vectors feature the encoded endoproteinase Factor Xa (pronounced “ten a”) proteolytic site that provides a way to separate the purification tag from the native protein. These vectors also carry a convenient multiple cloning region for ease in construction of fusion proteins. The system contains vectors in all possible sense reading frames, an avidin-conjugated resin, Streptavidin-Alkaline Phosphatase, a purification column and biotin. The PinPoint™ Xa Control Vector contains the chloramphenicol acetyltransferase (CAT) gene and is provided as a means of monitoring protein expression, purification and processing conditions.
Printed in USA. Revised 7/06.