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Promega Corporation

pGL2 Luciferase Reporter Vectors Technical Manual

Literature # TM003

The pGL2 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The pGL2 Vectors carry the coding region for firefly (Photinus pyralis) luciferase, used to monitor transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the pGL2 Vectors contain numerous features that aid the characterization and mutagenesis of the putative regulatory sequences.

Printed in USA. Revised 2/09.

 

PDF Icon Complete Protocol PDF (334kb)

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