MagneGST™ Protein Purification System Technical Manual
Literature # TM240
The MagneGST™ Protein Purification System provides a simple, rapid and reliable method to purify glutathione-S-transferase (GST) fusion proteins. Glutathione immobilized on paramagnetic particles (MagneGST™ Glutathione Particles) is used to isolate GST-fusion proteins directly from a crude cell lysate using a manual or automated procedure. The use of paramagnetic particles eliminates several centrifugation steps and the need for multiple tubes. It also minimizes the loss of sample material. Although the MagneGST™ System is designed for manual applications, samples can be processed using a robotic platform, such as the Beckman Coulter Biomek® 2000 or Biomek® FX workstation, for high-throughput applications.
The following change was made to the 8/14 revision of this document:
The composition of the MagneGST™ Binding/Wash Buffer was corrected to 2mM KH2PO4.
Printed in USA. Revised 8/14.