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Promega Corporation

GeneClip™ U1 Hairpin Cloning Systems Technical Manual

Literature # TM256

The GeneClip™ U1 Hairpin Cloning Systems consist of linearized plasmids designed for easy and fast cloning of hairpin target sequences to allow expression of siRNA target sequences in human cells. The plasmids contain the human U1 promoter, which allows transcription of the hairpin target sequences and generation of hairpin siRNA in vivo. The GeneClip™ U1 Hairpin Cloning System—Basic is intended for transient suppression of the gene of interest. The GeneClip™ U1 Hairpin Cloning System—hMGFP enables easy determination of transfection efficiency and allows selection of transfected cells by fluorescence-activated cell sorting (FACS®). The other GeneClip™ U1 Hairpin Cloning Systems offer antibiotic marker options(neomycin, hygromycin or puromycin) for selecting stably transfected eukaryotic cells so that experimental results do not depend on transfection efficiency.

Printed in USA. Revised 1/14.


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