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Promega Corporation

Erase-a-Base® System Technical Manual

  • Instructions for Use of Product(s)
  • E5750

Literature # TM006

The Erase-a-Base® System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA. The system is based on the procedure developed by Henikoff, in which exonuclease III (Exo III) is used to specifically digest insert DNA from a 5´ protruding or blunt end restriction site. The adjacent sequencing primer binding site is protected from digestion by a 4-base 3´ overhang restriction site or by an alpha-phosphorothioate-filled end.

Printed in USA. Revised 6/05.

 

PDF Icon Complete Protocol PDF (287kb)

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