Promega Corporation

ChipShot™ Indirect Labeling and Clean-Up System Technical Manual

  • Instructions for Use of Product(s)
  • Z4000

Literature # TM287

The ChipShot™ Indirect Labeling and Clean-Up System generates fluorescent cDNAs by synthesizing an aminoallyl cDNA and then conjugating the CyDye™ NHS ester to the cDNA. Either total RNA or poly(A)+ mRNA can be used with these systems. Resulting cDNAs are labeled to provide efficient microarray hybridization results. The ChipShot™ Indirect Labeling System includes a human Total RNA Positive Control to evaluate the performance of the synthesis, labeling and clean-up procedures. The ChipShot™ Membrane Clean-Up System produces highly purified, labeled cDNAs. Amplification of RNA is not required. All reagents are specifically optimized for microarray applications.

Printed in USA. Revised 3/09.

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