ChipShot™ Direct Labeling and Clean-Up System Technical Manual

Literature # TM286

The ChipShot™ Direct Labeling and Clean-Up System generates fluorescent cDNAs by direct labeling using Cy®3 and Cy®5 dCTP. Either total RNA or poly(A)+ mRNA can be used with these systems. Resulting cDNAs are labeled to provide efficient microarray hybridization results. The ChipShot™ Direct Labeling System includes a human Total RNA Positive Control to evaluate the performance of the labeling and labeling clean-up procedures. The ChipShot™ Membrane Clean-Up System produces highly purified, labeled cDNAs that are essentially free of unincorporated nucleotides. Amplification of RNA is not required. All reagents are specifically optimized for microarray applications.