Trypsin Gold, Mass Spectrometry Grade Technical Bulletin
Literature # TB309
Trypsin Gold, Mass Spectrometry Grade, has extremely high specific activity and is extremely resistant to autolytic digestion. Modified trypsin is maximally active in the range of pH 7–9 and is reversibly inactivated at pH <4. It is resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile and retains 50% of its activity in 2M guanidine HCl. The activity of trypsin is decreased when acidic residues are present on either side of a susceptible bond. If proline is at the carboxylic side of lysine or arginine, the bond is almost completely resistant to cleavage by trypsin.
Summary of Changes
The following changes were made to the 5/15 revision of this document:
1. The protocol steps in Section 3.B were revised.
2. The document design was updated.
Printed in USA. Revised 5/15.