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Promega Corporation

Proteasome-Glo™ Assay Systems Technical Bulletin

Literature # TB349

The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays that measure the three proteolytic activities associated with the proteasome (each of these three assays is available separately). The Proteasome-Glo™ 3-Substrate System provides three separate assays that differ in their ability to detect different protease activities based on their substrate components. The luminogenic substrates provided for the chymotrypsin-like, trypsin-like, and caspase-like activities are Suc-LLVY-aminoluciferin, Z-LRR-aminoluciferin, and Z-nLPnLD-aminoluciferin, respectively. Each substrate is added to a buffer system optimized for proteasome activity and luciferase activity to make a Proteasome-Glo™ Reagent for a particular catalytic activity. The individual Proteasome-Glo™ Reagent is added to test samples in an "add-mix-measure" format, resulting in proteasome-induced cleavage of the particular substrate. Substrate cleavage generates a "glow-type" luminescent signal produced by the luciferase reaction. In this homogeneous coupled-enzyme format, the signal is proportional to proteasome activity.

Printed in USA. Revised 9/11.


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