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Promega Corporation

ProteaseMAX™ Surfactant, Trypsin Enhancer Technical Bulletin

Literature # TB373

ProteaseMAX™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation. ProteaseMAX™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For in-gel protein digestion, ProteaseMAX™ Surfactant also improves the recovery of peptides. ProteaseMAX™ Surfactant can be used with existing in-gel or in-solution digestion protocols.

Summary of Changes
The following changes were made to the 2/15 revision of this document:
1. The instructions for adding TFA were made consistent between Section 4.A, Step 15, and Section 4.B, Step 8.
2. The document design was updated.

Printed in USA. Revised 2/15.


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