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Promega Corporation

PowerPlex® Matrix Standards, 3100/3130, Technical Bulletin

  • Instructions for Use of Product(s)
  • DG4650

Literature # TBD022

Proper generation of a spectral calibration file is critical to evaluate multicolor systems with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. The PowerPlex® Matrix Standards, 3100/3130, includes individual fragments labeled with four different fluorescent dyes. Each matrix fragment is in a separate tube: one tube contains a DNA fragment labeled with fluorescein (FL), one tube contains a DNA fragment labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein (JOE), one tube contains a DNA fragment labeled with carboxy-tetramethylrhodamine (TMR), and one tube contains a DNA fragment labeled with carboxy-X-rhodamine (CXR).

Printed in USA. Revised 6/13.


PDF Icon Complete Protocol PDF (4981kb)

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