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Promega Corporation

PowerPlex® Matrix Standards, 310 Technical Bulletin

  • Instructions for Use of Product(s)
  • DG4640

Literature # TBD021

Proper generation of a matrix file is critical to evaluate multicolor systems with the ABI PRISM® 310 Genetic Analyzer and ABI PRISM® 377 DNA Sequencer. To prepare a matrix, four standards are run under the same capillary electrophoresis (CE) or gel conditions that are used for samples and allelic ladders. The PowerPlex® Matrix Standards, 310, consist of DNA fragments labeled with four different fluorescent dyes: one tube contains DNA fragments labeled with fluorescein, one tube contains DNA fragments labeled with carboxy-tetramethylrhodamine (TMR), one tube contains DNA fragments labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxyfluorescein (JOE), and one tube contains DNA fragments labeled with carboxy-X-rhodamine (CXR).
Use the fluorescein Matrix, JOE Matrix, TMR Matrix and CXR Matrix for the blue, green, yellow and red standards, respectively.

Printed in USA. Revised 8/10.

 

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