MagZ™ Protein Purification System Technical Bulletin
Literature # TB336
Purification of a polyhistidine- or HQ-tagged protein that has been expressed in rabbit reticulocyte lysate is currently limited by hemoglobin in the lysate copurifying with the protein of interest. Hemoglobin copurification limits downstream applications (e.g., fluorescence-based functional assays, protein-protein interaction studies) and reduces the amount of protein purified. Thus, we developed a unique system that purifies polyhistidine- or HQ-tagged proteins expressed in rabbit reticulocyte lysate. These polyhistidine- or HQ-tagged proteins are 99% free of contaminating hemoglobin. The MagZ™ Protein Purification System provides a simple, rapid and reliable method to purify expressed polyhistidine- or HQ-tagged protein from rabbit reticulocyte lysate. Paramagnetic precharged particles can be used to isolate polyhistidine- or HQ-tagged protein from 50–500µl of TNT® Rabbit Reticulocyte Lysate with minimal copurification of hemoglobin.
Printed in USA. Revised 6/09.