This country code will remain if no action is taken to change it.
Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.
Instructions for Use of Product(s)
Literature # TB336
Purification of a polyhistidine- or HQ-tagged protein that has been expressed in rabbit reticulocyte lysate is currently limited by hemoglobin in the lysate copurifying with the protein of interest. Hemoglobin copurification limits downstream applications (e.g., fluorescence-based functional assays, protein-protein interaction studies) and reduces the amount of protein purified. Thus, we developed a unique system that purifies polyhistidine- or HQ-tagged proteins expressed in rabbit reticulocyte lysate. These polyhistidine- or HQ-tagged proteins are 99% free of contaminating hemoglobin. The MagZ™ Protein Purification System provides a simple, rapid and reliable method to purify expressed polyhistidine- or HQ-tagged protein from rabbit reticulocyte lysate. Paramagnetic precharged particles can be used to isolate polyhistidine- or HQ-tagged protein from 50–500µl of TNT® Rabbit Reticulocyte Lysate with minimal copurification of hemoglobin.
Printed in USA. Revised 6/09.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.