Instructions for Use of Product(s)
Literature # TB552
Luciferase reporter assays are used widely to investigate cellular signaling pathways and as high-throughput screening tools for drug discovery. The GloResponse™ 9XGAL4UAS-luc2P Cell Line is a clonal derivative of Human Embryonic Kidney 293 (HEK293) cells. These cells contain nine repeats of GAL4 UAS (Upstream Activator Sequence). This sequence drives transcription of the luciferase reporter gene luc2P in response to binding of a fusion protein containing the Gal4 DNA Binding Domain (e.g., the Estrogen Receptor Ligand Binding Domain in pBIND-ERα Vector [Cat.# E1390]) when activated by a ligand.
The GloResponse™ 9XGAL4UAS-luc2P Cell Line was generated by clonal selection of HEK293 cells stably transfected with the pGL4.35[9XGAL4/Hygro] Vector (Cat.# 1370). This cell line incorporates the improvements developed for the pGL4 family of reporter vectors for enhanced performance. The destabilized luc2P luciferase reporter is used for improved responsiveness to transcription dynamics. The luc2P gene is codon-optimized for enhanced expression in mammalian cells, and the pGL4 plasmid backbone has been engineered to reduce background reporter expression.
Summary of Changes
The 4/15 version of this Technical Bulletin was revised to update patent and disclaimer statements.
Printed in USA. Revised 4/15.
Experienced User Protocols