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Promega Corporation

Caspase-Glo® 6 Assay Technical Bulletin

Literature # TB366

The Caspase-Glo® 6 Assay is a homogeneous, luminescent assay that measures caspase-6 activity. The Caspase-Glo® 6 Assay provides a luminogenic caspase-6 substrate, Z-VEID-aminoluciferin, in a buffer system optimized for caspase-6 and luciferase activities. Addition of a single Caspase-Glo® 6 Reagent in an “add-mix-measure” format results in caspase-6 cleavage of the substrate and generation of a glow-type luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the luminescent signal is proportional to the amount of caspase-6 activity present. The Caspase-Glo® 6 Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) that is formulated to generate a stable glow-type luminescent signal across a wide range of assay conditions.

Summary of Changes
The following changes were made to the 3/15 revision of this document:
1. The patent/license statements were updated.
2. The document design was updated.

Printed in USA. Revised 3/15.


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