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Promega Corporation

Caspase-Glo® 2 Assay Technical Bulletin

Literature # TB365

The Caspase-Glo® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity.The Caspase-Glo® 2 Assay provides a luminogenic caspase-2 substrate, Z-VDVAD-aminoluciferin, in a reagent optimized for caspase-2 and luciferase activity. The VDVAD pentapeptide sequence is an optimized recognition sequence for caspase-2, although this sequence also is an acceptable substrate for caspases-3 and -7. A single Caspase-Glo® 2 Reagent is added to test samples, resulting in caspase cleavage of the substrate and generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to caspase activity.

Summary of Changes
The following changes were made to the 3/15 revision of this document:
1. The patent/license statements were updated.
2. The document design was updated.

Printed in USA. Revised 3/15.


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