We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Wizard® DNA Clean-Up System Technical Bulletin

  • Instructions for Use of Product(s)
  • A7280

Literature # TB141

Concentration of DNA samples, removal of small nucleotides from reactions and purification of DNA from proteins or salts all require organic extractions and precipitation, which can result in low and variable recoveries. The concern over recovery increases when working with amounts of DNA in the 50–500ng range.
The Wizard® DNA Clean-Up System provides a simple and effective way to purify linear and circular DNA (200–50,000bp) from the following:

  • restriction enzymes, including heat-stable restriction enzymes
  • phosphatases and kinases
  • DNA polymerases, including Taq DNA polymerase
  • exonucleases and endonucleases, including DNase I but not RNases
  • mononucleotides
  • salts

The entire procedure can be completed in 15 minutes or less, with no organic extractions or ethanol precipitation. DNA is eluted from the Wizard® Clean-Up Resin in water or TE buffer, ready for use.

Printed in USA. Revised 12/10.


PDF Icon Complete Protocol PDF (96kb)

PDF Icon Quick Protocol PDF (86kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.