We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Prime-a-Gene® Labeling System Technical Bulletin

  • Instructions for Use of Product(s)
  • U1100

Literature # TB049

The Prime-a-Gene® Labeling System is based on the method developed by Feinberg and Vogelstein, in which a mixture of random hexadeoxyribonucleotides is used to prime DNA synthesis in vitro from any linear double-stranded DNA template. With this method it is possible to generate probes of extremely high specific activity (>1 x 109 cpm/µg), even using DNA fragments cut from agarose gels. Since the input DNA serves as a template and remains intact during the reaction, minimal amounts of DNA (25ng) can be labeled to a high specific activity. Typically, greater than 60% of the labeled deoxyribonucleotide can be incorporated into the Lambda Control DNA using the labeling reaction described here. Incorporation may vary from 40–80% with other samples, depending on the template and reaction conditions used. Using a template greater than 500bp, probes generated with the Prime-a-Gene® Labeling System are generally 250–300bp in length and are suitable for a variety of applications.

Printed in USA. Revised 12/12.

 

PDF Icon Complete Protocol PDF (403kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.