pGEM®-3Z Vector Technical Bulletin
Literature # TB033
The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ alpha-peptide and multiple cloning region arrangement from pUC18. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. This arrangement gives rise to a functional alpha-peptide that is capable of complementing the product of the lacZDeltaM15 gene to produce functional beta-galactosidase. Cells with the genotype lacZDeltaM15, and also containing the pGEM®-3Z Vector, will be blue in color when plated on indicator media containing IPTG and X-Gal. However, when the lacZ alpha-peptide sequence is disrupted by cloning into the pGEM®-3Z multiple cloning region, complementation does not occur and no beta-galactosidase activity is produced. Therefore, bacterial colonies harboring recombinant pGEM®-3Z Vector constructs remain white.
Printed in USA. Revised 5/09.